Food-allergic children have decreased peripheral regulatory B cells and altered B cell responses to IL-10

Increases in IL-10-producing cells and allergen-specific IgG4 are associated with allergic tolerance, including tolerance to foods; however, B cell responses IL-10 the role of regulatory, have not been well-explored context food allergy. Blood samples were collected from food-allergic (FA) non-food-allergic (NFA) children between 2 6 years age. Flow cytometry was performed determine frequency receptor (IL-10R)+ IL-10+ cells. Peripheral blood mononuclear (PBMCs) purified cultured combinations anti-CD40, IL-4, IL-10. Immunoglobulins quantified culture supernatants using multiplexed immunoassays. With data collection analysis ongoing, preliminary results show decreased IL-10R expression on memory FA compared NFA (p < 0.02). Both display low frequencies periphery. among CD38+ CD27+ 0.03), emerging trends other subsets. IL-4-induced production PBMCs 0.01), but children. also increased IgE = 0.05). These suggest that, comparison children, a ability bind IL-10, as well an altered response respect production. peripheral regulatory